辽宁花生疮痂病病原学及侵染特性研究.docx

,塔修茨孝人冬论文题目：辽宁花生疮痂病病原学及侵染特性研究独创性声明本人声明所呈交的论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除了文中特别加以标注和致谢的地方外，论文中不包含其他人已经发表或撰写过的研究成果，也不包含为获得沈阳农业大学或其它教育机构的学位或证书而使用过的材料。与我一同工作的同志对本研究所做的任何贡献均已在论文中作了明确的说明并表示谢意。研究生签名:时间：年月日导师签名:时间：年月日关于论文知识产权和使用授权的说明本论文的知识产权为沈阳农业大学所有。本人完全了解沈阳农业大学有关保留、使用学位论文的规定，即：学校有权保留送交论文的史印件和遨盘，允许论文被查阅和借阅，可以采用影印、缩印或扫描等组制手段保存、汇编学位论文同意沈阳农业大学可以用不同方式在不同媒体上发表、传播学位论文的内容。学位论文中的所有内容不经沈阳农业大学授权不得以任何方式擅自对外发表。（保密的学位论文在解密后应遵守此协议）研究生签名:时间：年月日导师签名:时间:年月曰CONTENTSAbstract1Abstract(inEng1.ish)3Chapter I Researchprogressinfunga1.diseasecausedbySphace1.omas51 CharacterizationandC1.assificationofSphaee1.omaspp52 Typica1.symptomsofdiseasecausedbySphace1.omaspp52.1 Citrusscab52.2 Grapeb1.ackpox62.3 Supcr-c1.ongationdiseaseincassava62.4 Peanutscab72.5 Otherfunga1.diseasecausedbySphace1.omaspp73 Theoccurrenceru1.esofdiseasecausedbySphace1.omiispp84 ThepathogenicmechanismofSp1.uice1.omaspp94.1 E1.sinochrome94.2 Gibbere1.1.in1()4.3 Resistanceofcu1.tiv<rsIOChapter II Occurrenceandetio1.ogyofpeanutscabin1.iaoningprovince12Section 1 Thesurveyofdiseaseandidentifitionofpeanutscab121 Materia1.sandmethods131.1 TheSUrVCyofdiseaseofpeanutscabin1.iaoningprovince131.2 Iso1.ationandpurificationofpathogenic131.3 Pathogenicitytest131.4 Identificationofpathogenic142 Resu1.tandana1.ysis152.1 DiseaseSynIP1.OmSofpeanutwrab152.2 Diseasesurveyandcharacteristicsofoccurrence162.3 Iso1.ationofpathogeneticfungi172.4 Resu1.tofpathogenic182.5 Pathogenidentification183 Conc1.usionandDiscussion24Section 2 Phenotypicandmo1.ecu1.archaracterizationofS.arachidisin1.iaoningprovince261 Materia1.sandmethods261.1 Strainsfortest261.2 Themorpho1.ogica1.observationandcu1.tivatecharacteristics281.3 TheMo1.ecu1.arCharaCIeriStiCS282 Resu1.tandana1.ysis292.1 Thephenotypiccharacterization292.2 Themo1.ecu1.archaracterization313 Conc1.usionandDiscussion35ChapterII!Resistanceidentificationofpeanutscabfordifferentcu1.tivatedvarieties371 Materia1.sandmethods371.1 Materia1.fortest371.2 Inocu1.ationmethod371.3 Testsiteset371.4 Investigationmethod38CO1.HCntS1.5 Re1.ativeresistanceeva1.uationstandard382 Resu1.tandana1.ysis382.1 Resu1.tofvarietyresistanceidentiiicationofpeanutscab382.2 Varietyresistanceindexana1.ysisofvariance403 Conc1.usionandDiscussion40Chapter IV Thepre1.iminaryinfectionssourceandtempora1.dynamicofepidemicofpeanutscab421 Materia1.sandmethods421.1 Materia1.fortest421.2 PrimaryinfeCIionsourceOfPeanUtscab421.3 Partsofpathogeninfection431.4 Tempora1.dynamicofepidemicofpeanutscab431.5 EtTectofcanopyconditionsonepidemicOfpeanutscab432 Resu1.tandana1.ysis432.1 Resu1.tofprimar)'infectionsourceofpeanutscab432.2 Resu1.tofpartsofpathogeninfection452.3 Seasona1.epidemiccurveofpeanutscab452.4 Mode1.fbrtempora1.dynamicofepidemicofpeanutscab472.5 Effectofcanopyconditionsonepidemicofpeanutscab483 Conc1.usionandDiscussion49Chapter V InfectioncharacteristicsofpeanutS.arachidis51Section 1 InfectionsconditionsofS.arachidis511 Materia1.sandmethods511.1 Materia1.for1.estandinocu1.ationmethod511.2 Effectsoftemperatureontheinfectionofthepathogen511.3 Effectsofwetnessdurationontheinfectionofthepathogen511.4 Effectsoftemperatureandwetnessdurationontheinfectionofthepathogen.511.5 Efiectsofcu1.turetimeontheinfectionofthepathogen512 Resu1.tandana1.ysis522.1 EITectsOftemperatureontheinfectionofthepathogen522.2 Effectsofwetnessdurationontheinfectionofthepathogen522.3 Effectsoftcnpc11turcandwetnessdurationontheinfectionofthepathogen.532.4 Eficctsofcu1.turetimeontheinfectionofthepathogen533 Conc1.usionandDiscussion54Section 2 InfectionprocessofS.arachidisandphysio1.ogica1.responsestopathogeninfectioninpeanut561 Materia1.sandmethods561.1 Materia1.fortestandinocu1.ationmethod561.2 MicroscopicobservationoftheinfectionprocessofS.arachidis561.3 Extractionand1.estofactivityofhostdefensiveenzyme572 Resu1.tandana1.ysis572.1 InfectionprocessofS.arachidis572.2 Effectofpathogeninfectiononhostdefensiveenzyme593 Conc1.usionandDiscussion64References65Tothank71PubIishionduiingtheperiodofstudyforamaster'sdegree72表2-5进化树中菌株来源及GenBank序列号Tab1.e2-5OriginandGenBankaccessionnumber*ofreferencestrains位株编号S(Hiii)No.曲楂幺秋Name所属地1.cica1.i1.yCknBankNo.rrs1.SUSSURPBISphacf1.tffJiaarachidisCK的33Brazi1.JN9434B5JN940374JNg40548JN992619CIjC18529Brazi1.JNM3&UJNM>372JNy40547JN99261KE1.xinoefdwei1.iiCK18570Brazi1.JNW503JNgO385JN94O565JN992654CPe1K535Brazi1.JN9434%JNWO382JNg4O5S9-S-CrythriiKteCPC18530Brazi1.JVM3502JN<M0W2JN94O566JN992636CK：18542Brazi1.JN<M34X7JN<M(W<JNg40550JN99262ICrC18540Brazi1.JVM34«6JMgO389JNy40549JN99262()ENerben比CPC18561Brazi1.JNW3499JNMoS91JN9