愈伤诱导和继代条件优化.docx
nhighefficientca1.1.usp11>1.iferatonprotoco1.andIimonoidsandDictnmnineaccumu1.ationofDictamnusdasycarpusTiircz.,ava1.uab1.emedicina1.p1.antinChinaCa1.1.usinduction,pro1.iferationfrommature1.eafcxp1.antsand1.imonoidsaccu1.ationofDictanmtsdasycarpusAbMn1.CiDkvwwiwvdasycrptisTurczIsapcrennia1.va1.uedherbintbcRacracfami1.y.Inthisstudy,ahighCfneSenIca1.1.usinductionandpr。IifCnHionrocoifromyoungstemcxp1.antsofDicu(Sanyaiqwha<.beenCMiibIishedand成分含量.Ca1.1.uswasinitM1.FrImtbcynungMcmcxpbntsiubiitcdonMr<higcKnC1.SkoOg(MS1.mediumUippkmcnccdwithdicrcnp)ungrowthrcgu1.uu)11.虐伤情导In1.hed1.rvntCuiicen1.ra1.iiintreaUnen1.s,MAA.IBAatx1.2.4-DcmMS.dieUiduc1.ioneffect%s!>sedxignficamUi1.Yervn1.m1.e.11ehighestUidixIiDnfvqey(M)O%)WkWIknVedcu1.1.swnsobscn,cdonMSmediumsupp1.ementedwith1.0mg.,1.X4-D拿伤TDZinCombeiwiionwithNAAWaSverysuitab1.eforCaHsro1.itctii(ioncomparedioTDZinCOmtMnaiMMiwith2.4-0<×TOZincombtnatowithIBA.Freshanddrywe砌ofca1.1.uscu1.turedonMSmediumsupp1.ementedwith0.5mg<1,KTincombinationwith2.0mg,1.NAAwa、8.2gand23gwi11(hin35dayscu1.ture,respective1.y.Andthegmwthindexreachedto120.1Quaniitaiiveana1.ysis1.'IiitMMiuidsbyHP1.CHhOW1.1.O1.IhehyIochemicaiprofi1.eofca1.1.usCu1.tuiudc>MSediunMippaenicii1.edwith0.Smg,1.Kinc<mbta(i<>nvi1.h2.0ng<,1.NAAWXsimi1.;ir<othatofwi1.dphnKandtbccontentof<)MVum<.rra¾indk<M(WM).1.i11HnintntV*WaS"*"and."mggDWcomparedtothatof*,and*mg2DWinwi1.dPtmIs.白色食仿I1.m!m»ho*rdrvhti1.y1.nwpr>1.it*m1.i<m川th。”Rh<an11.obtainedw*whheandfrbb1.e.Keywords:DidMCUSdawErPux、Ca1.hwir1.c(ion.Ca1.1.1.npro1.iferation,1.imnn<M<hAbbreviationsBAP6-RcnzyhiniifKipunecNAA-rup1.hancJE1.iCvidWkIMMf1.1.j：UtEIi1.<«idB4n1.o(u1.fwfifrac)xi.CmZwyrmXq*sr.Gmhtoyg1.IMeyhw:3)IO2J1.xt<<C«TV»Mdn»».<>><xktm1.ammroMi%kycfr>*eftMoi1.fkDkuru¼U¼y<tfpuRwdaeMitry.IO7<5.I3)M2OT.2(MKTTDZThKiMJMnMiN-hc11y1.-N,-1.2.34hiadiazo1.-5-y1.urc1,DZ>2D2。沁hSkpboxyMreiMacidMSMuniihigcandSkggftttIMXf1.；”KimMKimHS>nIaGGmAnW.C1.”SI:A««iSHMnrttfnry11MHeofDkurnud*y<iftTu/.fo)1.N«1JIiefme3ickf<J<ikm1.uvdby痴in>fkjIinixnArrwakc.JJhuf4u11uc<tfXI).14247I-177.21XK-G11p>*nwQIr<h»vwyjri.*im*ni<a1.>p-MK5ang.Xuan.1.J.XuY.MAZang.J.S.2002.Cyktoxc103Qgdandren*no944×WMenOSC，搂r11ntherootberk<fDkm11usjCftMSAYnB1.Introduction白itt1.介：Diitamrnts/nruavTurczisaperennia1.hcd½dp1.antintheRutaceaefami1.y,ada1.vk>EaMAsia.'hercHbarkofdiePIanaisca1.1.edCitrtexJicfamniintheQieePAIrnIaCrPneEwhichKIKCdinChinese1.rddition1.mcikri11cforIbcIrcatmcntofjaundice,cough,r1.un½tism攸iBm1.SOmCindicde才小H1.有效成分娥及其畲二，*tt1.jBMI*'201VSihAi4c>(5dc<<a(ukoof1.iauM.Jk1.amiiH<4)auwcMXIGaijndknc<HHPManybk>1.ogica1.1.yJtctivccocnponcntshavebeeni加Mifiedfromthe11×xbarkofI),datyrarpusschas1.imonoids,furoquino1.inca1.hitoids.f1.avonoids,coumarins,sesquiterpenes,scquitcncg1.ycoMde.andptc11uhcg1.ycmitk'47收避AmDIttUw1.n.1.imonoid,diet;IJ1.Im1.-ObiHr1.nX>m:XHM1.Iiuxine1.kKM!areIhCnnMdetiMmWEedrepreenuive«11.iMM*：-N06PEtrgJIuMCMUTi1”o1.k*AtdbyhifiiperkotiMM风向CtgM(UwIyfc<cV1.ennn«1.>(«i*X、CaMtiwatp,u*1.*isCvCteGDktSrnIiEf<活性成分功效Manystudieshavebeenconductedonpharmnco1.ogica1.activitiesof(hefourconsticucs.*ttImMBV-YeCC.v.C.Km.S.H.Km.M.J.Yang.HJ.Rh>uMR.&ParXJ.H.<2015).1.ircnin&Ccmpoooni“(XttmniRacisC«>e«.E<41.nCuwdCekiumMdCAwPI广IM1.IMW；XW-Neirvrt>xtic1.k<u1.o(R*«»tBMkofIXt1.imiMdMbV4*.pdf3XC11wpMrt*0<Q1.f*mwinixtiatfyof3Eu1.ni1.fmmDktXnWqh<11rppdf拧置苦素的活性1.imonin.oneofthemajorCompooetsfoundinthep1.anu1.,RuiaceaeandMcIiACCiieOimiHcx.h;tb<xnsbc>wncop1.ayvariniwbk>ng>ca1.m1.cincancer,inammatiix).a11<1.obcshyinmanydifferentCd1.卜PCSand1.is、UaJnd3PmySSqiunifkumMUrfroicvii、Cacii、kicagainsg1.uanwc-iduc<xincurocoxiciymprimaryCUkUrCgofra1.xti1.CdkIh1.1.*ttIMMUt*201S-D*ruryohuu<e*ftf411r<an1.inub*nBM<c>90tn>fty.IM«1fcytriyvrrm*mdnb<witythmfTGR5JrejPPARypdv>y.pj(p-ManyMudiexhavebeenrePwtedth；uobiicun<>newasfoundtnjrvat1.yenhanceIheCy1.otoxicicyOfmicr<Mub1.cinhibiton;.toinhibitIhcprdifera1.innoftumorce1.1.%byifx1.cin!apoptosis,undtosuppresseshyperg1.ycemianndobcsii>1.II1.档活性HuxiiieIkMieexhibit%avanv1.yofin1.vcMnvn>X!rties.inc1.udingIbean1.inIIainina1.orvbowe1.disease.DCUmPm1.CC1.ive,andimcctic1.a1.actiitie<rrwdndk>nc.MEIMMtt二X1.1.UmdUah1.”>Jv<1.b.De1.mcn1.EfYeUt4I4tie1.k<e(nmk1.M11tu*ryc><apMR”irM>jcrMmWf3)I6-Wcw1.(<djpert(r<nBWWKttDictamninc.anatura1.p1.antp<duci.hasbeendcmonstrutod(ohavesignifkuntCfTOe1.Sonan1.i-p1.a½k<JFKreWin.uxau1.ar-rtfhinj:.an<itxWri1.antifunga1.ac1.ivMics；)Dk(amniH.Dk1.antfiinehaohobevnwide1.yUMXJk»Irca1.certainJcindisea>cs.Kft1.ft'"''Co.”.t<(¼Mrfin皿rcm>11*wd%<<jurmirw,»r>r*h>ti%v0MT<xw1m1fnmIktf11>ihrkof>ixmg><vp<1.1.nrKucwc)gInconc1
