ISO TS 04985-2023.docx

ISO/TSTECHNICA1.4985SPECIFICATIONIDF/RM255Firstedition2023-07Mi1.kandmi1.kproductsDeterminationofa1.ka1.inephosphataseactivityF1.uorimetricmicrop1.atemethodIS(Jff6icncc49H511htt(E)IDF/RM255：2023(E)COPYRIGHTPROTECTEDDOCUMENT©ISOandIDF2023Mrtf1.hreduscftvdi1.HikwjkNhCrWiminapviiiim1.>rbyqmFitttacmh8>>ni<w4cUMnbEdtne崛qniN911hi6cnpyip<bMMUntionmaytheinternetoranint11net1.withoutpriorwrittenpermission.PermissioncanberequestedInxncitherISOattheaddressbe1.oworISO'smemberbodyintheCoUntryOfthCrequester.fib包酬丽ndonnet8CH1214Vcmicr,GenevaPhone：*41227490111Emai1.：CoPyrh田EErgWebsitc八,.7,so.o11gsff1.Ht1.d三EaWM够UonReyerS70/BB1030Brusse1.sPhone：*3223256740Fax：*3223256741Emai1.：MEHWebsite：www.ft1.dfx>rgPub1.ishedinSwitzer1.and©ISOandIDF2023-A1.1.nghtsreservedContentsForewords1010.2.2Cheescsamp1.es.12AnnexB(informative)Methodcomparison8.1.3A1.ka1.inephosphatase-freecheese9.1.3Qua1.ity1.inearity-69.2.3Preparationoftheca1.ibrationstandard._.一.69.3.3Cheesesamp1.es8iii8.1.1Genera1.8.1.2PageScopeNormativereferences一.11Termsanddcnitions.1Princip1.e1Reagents.2Apparatusandmateria1.s3Samp1.ingPreparation一448.1PreparationOfa1.ka1.incphosphatase-freesamp1.e.448.2Preparationoftestsamp1.e»«««t«Mt(M(WtM(M(«(*Mt(««««Iteett55ProcedureS9.1Instrument9.1.1Instrumentsettings59.29.1.2Testthecontro1.Ca1.ibrationrangeoftheinstrument69.2.1Genera1.i*M(i21M*MM>4i*M*MMaMM2*9.3Determinationworkingcurveso1.utions79.3.1Mi1.ksamp1.es79.3.2P1.ate1.ayoutandf1.uorescencemeasurement9.4Testsamp1.e-re1.atedcontro1.s99.4.1Recommendednegativeandpositivecontro1.s9Ca1.cu1.ationandexpressionofresu1.ts1010.1 Ca1.ibrationcurve1010.2 Ca1.cu1.ationofA1.PactivityOfunknownsamp1.es11Mi1.k10.2.3Ca1.cu1.ationexamp1.e1310.3 Expressionofresu1.ts14Precision1411.1 Inter1.aboratorytest1411.2 2Repeatabi1.ity-14TestreportISAnnexA(informative)Precisiondata16Bib1.iography.21©ISOandIDF2023-A1.1.rightsreservedForewordsstandardsthrough(ISOISOmittees.workofPreparingbody1.ntemationa1.Standardsisnorma1.1.ynatterswork.e1.ectrotechnica1.StandardizationAheInternationa1.E1.ectrotechnica1.Commission(IEC)ona1.1.describedtypesofISO/IECDirectivesrShouIdbeParHCU1.ar,documentwasapprova1.accordancewiththeISO（八）patent(三).attcntion1.akcs1.hcnopossibiHtyconcerningi11p1.enentationvahdityapp1.icabi1.ityinvo1.vcc1.aimedcautioncdavai1.ab1.cWWW0iwg/PaUHKdatcstinformation,Whichresponsib1.eidentifyinganypatcntconstitutenameendorsement,documentisinformationgivenfortheconvenienceofusersanddoesnotexpressionsre1.ated。伸nization(WTO)assessment,asinwe1.1.asinfomationBaierstoTradeadherenceseeThisdocumen1.protucf5preparcdIntcrnationa1.ComrnitteeFcdcra1.ion(IDF).¾ocproductsSubcommitteebySC1.SOcomp1.eteIistingquestionshodiesthiscandocumentWwwTki<herg三effiberstH)natiQna1.standardsbody.AOandIDF2023-A1.1.11ghtsreservedISO(theInternationa1.OrganizationforStandardization)isawor1.dwidefederationofnationa1.carriedoutbodiesmemberbodies).TheEmitteehasbeenestab1.ishedhastherighttoberepresentedonthatcommittee.Internationa1.organizations,governmenta1.andnon-governmenta1.,in1.iaisonwithISO,a1.sotakepartintheofISOco1.1.aboratesc1.ose1.yvrithTheproceduresusedtodeve1.opthisdocumentandthoseintendedforitsfurthermaintenancearedifferentinthedocumentPart1.Innoted.ThiSthediffereddraftedincriteriaforeditoria1.ru1.esoftheISO/IECDirectives,Part2(seewww.iso.org/directives).vofdrawsISOtopositionthatthetheevidence,ofthisordocumentmayofanytheusepatentrightsinrespectthereof.Asofthedateofpub1.icationofthisdocument,ISOhadnotreceivednoticeof（八）PatCnt(三)whichmayberequiredtoimp1.ementthisdocument.However,imp1.ementorsaredatabasethatthisatmaythe.ISOsha1.1.behe1.dmayforobtainedfromtheora1.suchpatentrights.AnytradeusedinthisForanexp1.anationofthevo1.untarynatureOfstandards1.themeaningofISOspecifictermsandtheWor1.dTradeconformityprincip1.estheTechnica1.about1.SO*s(TBT)1towww.iso.org/iso/foreword.htm1.Mi1.kandmi1.kwas,andthebyTechnica1.DairyISO/TC34rItisbeing,pub1.ishedjoint1.y5,andIDF.Anyfeedbackoroftheseonbefoundatshou1.ddirecteduser's.1.fiF<(thHiMn1.MHCnaIStfihiiy)IderSFdd呷Ift1.Pn)IhItgiOb中，。位IpXU油ectnembe*sn曲agi1.&u&pmiahtiMtheCommittees,whicharenationa1.associationscomposedofrepresentativesofdairy-re1.atednationa1.interestgroupsinc1.udingdairyfanners,dairyprocessingindustry,dairysupp1.iers,academicsandgovernments/foodcont