ISO 04484-2-2023.docx

INTERNATIONA1.STANDARDISO4484-2editionFirst202309Texti1.esandtexti1.eproducts一Microp1.asticsfromtexti1.esources一曲用茎ativeandquantitativeana1.ysisofmicrop1.asticsTexti1.esetproduitstexti1.esMicrop1.astiquesd,originestexti1.es一Partie2:Ana1.ysequa1.itativeetquantitativedesmicrop1.astiquesReferencenumberISO448422023(E)COPYRIGHTPROTECTEDDOCUMENT©ISO2023IUirhM*hedbdi1.iUedotherwiseupdhi.or啪UIBndttaeDmk<nroni(ncm11ni10tf1.*Mqn1.C6pW11opypMRHt1.onmaytheinternetoranintranet,withoutpriorwrittenpermission.PermissioncanberequestedfromeitherISOattheaddressbe1.oworISO'smemberbodyinthecountryoftherequester.f),WV>fifiHipB1.andonnet8CH-1214Vernier,GenevaPhone：M1.22749O1.11觥ftte:丽丽BQrgPub1.ishedinSwitzer1.and©ISO2023-A1.1.rightsreservedO2023-A1.1.rightsreservedThereissignificantevidencethatthetexti1.esectorre1.easesmicrop1.astics(MPs)intotheenvironment.sizebewhenpresentinfortheassessmentoftheirthebiota,andso,and,number,shapethedeve1.opmentofacountingtechniquecanbeahe1.pfu1.approach.toidentifyandofthethemfromana1.ysedarenotofThisdocumentisdesignedtoprovidethenature,numerica1.concentration,surfaceareaand(estimated)massthematrices.vDependingonthematrice,pre-treatmentofthesamp1.eisnecessarytoconcentratetheHiicrop1.asticsande1.iminateThisandorganic(forexamp1.ebio1.ogica1.)observationofthecanbyanwithmicroscope(OM)andthenidentificationofthemicrop1.astics(MPs)bymo1.ecu1.arspectroscopy.Thisdocumentprovidesthepossibi1.ityofusingtwodifferenttechniquesofmo1.ecu1.arspectroscopy,Micro-Thisdocumentisdesignedtoa1.1.owthere-eva1.uationofmicrop1.asticcountingdatawhentoxico1.ogica1.andenvironmenta1.impactindicationsbecomeavai1.ab1.e.anddocumentdescribestheofofana1.ysisforresu1.tfromtheerrorsinthedifferentfi1.tersimp1.ythatrep1.icatesshou1.dbeperformedtoestab1.ishprecision.betakenintoaccountforausefu1.information(e.g.assessmentofshape.andcomposition,etc.)thatcanItiswe1.1.knownthatsomeInicrop1.astics(MPs)areIipophi1.eandcanbevehic1.esfortoxiccompounds(e.g.PCBs,PAHsrdioxins)orvehic1.esofpathogenicmicroorganismsadheredtotheirsurfaceandcanbeThesourcesofmicrop1.asticsarenumerous.Theirshapesandsizesarea1.sovariab1.e.Inthecaseofthosere1.easedbytexti1.es,thetypica1.(butnottheon1.y)morpho1.ogyisfibrousandtheirdiameterand1.engtha)Effectofmicrofi1.amentsb)Effectofso1.utionc)EffectofconcentrationKeyYprobabi1.ityZso1.utionm1.Wconcentration(microfi)aments1.)FigureB.3-Effectofthenumberoftheoretica1.micro1.amentsrvo1.umeandconcentrationonthedetectionprobabi1.ityofthesing1.emicrofi1.amentTab1.eB.2Estimatedprobabi1.itysurfacesfordifferentmateria1.stestedandtheirstandarderrorsTheoretica1.micro1.amentsSuspensionvo1.ume(m1.)Concentration(m1.crof1.1.aments/1.)Po1.yamicic6:detectionprobabi1.ityPo1.yamidc6,6:detectionprobabi1.ityPo1.yester:detectionprobabi1.it>>Po1.ypropy1.ene:detectionprobabi1.ity1805003600288,9270f92S(0z00487J(O,OO928)(0.00594)(0,00995JTab1.eB.2(continued)Theoretica1.micro111.a-mentsSuspensionvo1.ume(m1.)Concentration(microf1.1.a*ments/1.)Po1.yamidc6:detectionprobabi1.ityPo1.yamidc6,6:detectionprobabi1.ityPo1.ycstcndetectionprobabi1.ityPoiypropy1.ene:detectionprobabi1.ity2569002840.9000.9351).919D,934(6,00494)(6,00846)(0,00506)(6,00834)Xprobabi1.ityYtheoretica1.microfi1.amentsZso1.utionm1.Figure B 4Estimatedprobabi1.itysurfacesfordifferentsjrntheticmateria1.susedAnnexC(informativeExamp1.eofc1.assificationofMPs6mnrithk3tovopynqem1.cdfofhahdndhridftac)nofmicropartic1.esco1.1.ectedonace1.1.u1.osefi1.terbyFigure C 2showsanexamp1.eofautomaticimageana1.ysis(6.17)ofMPsco1.1.ectedonasi1.iconfi1.terbyN11gjFJIR(6.1).MPchssica1.ionintermsoftheirpo1.ymergroup,positioncoordinates,dimensionsFigureC.1.Examp1.eofmanua1.c1.assificationofmicropartic1.esco1.1.ectedonace1.1.u1.osefi1.terbyoptica1.microscopy帘即ms的小SHUHm1.CaSC6dnp3叫IriiHobNiitt山蜂i1.4ttmahM*sisio6fmkhdg)h必mbtkmpRdinanFigureC.3-Micro-Ramancountingschemica1.identificationanddimensiona1.distributionofMPpartic1.es(diameter)co1.1.ectedonasi1.iconfi1.ter1(2HI5678i1.(111213旬34Bib1.iographyISO137,Woo1.Determinationoffibrediameter-ProjectionmicroscopemethodiQW:1997,Waterqua1.ityDeterminationofsuspendedso1.idsbyfi1.trationthroughg1.ass-夕MDeterminationofthechemica1.oxygendemandindex(ST-COD)ISO/TR11827:2012,Texti1.esCompositiontestingIdentificationoffibresISO/TR21960:2020,P1.asticsEnvironmenta1.aspectsStateOfknow1.cdgeandmethodo1.ogiesftN喃社如的Mir娴i1.(i也成ffif躺烈:e呻曲丽树IfijrtjIedeterminationOftheconc