PBS铁氰化钾缓冲液的配制.docx

PBS冲液的配制方法一：母液的配制：0.2MNa2HPO4:称取71.6gNa2HPO4-12H2O,溶干100Oml水0.2MNaH2PO4:称取31.2gNaH2PO4-2H2O,溶于100oml水各种浓度PB(PH7.4)的Je制：先配0.2MPB(pH=7.4,100ml)：取19ml0.2mol1.的NaH2PO4,81ml0.2mol1.fi¾Na2HP4o然后只需将02MPB(PH=7.4)按相应比例适当稀释:取50ml0.2MPB,加水稀释至100Ornl即可(0.01MPB(PH=7.4)若须要NaCl的话，加入NaCl至0.9%(g100ml)即可。方法二：称取NaCI8g,KCl0.2g,Na2HPO412H2O3.63g,KH2PO40.24g,溶于90Oml双蒸水中，用盐酸调PH值至7.4,加水定容至11.,常温保存备用。PBS-T缓冲液含0.05%吐温-20的pH7.4的磷酸盐缓冲液(简称为PBS-T)PBS-T缓冲液MX300lTween-20加入PBS100mI中，混匀后即刻运用。现用现配。PBS缓冲液一般作为溶剂，起溶解爱护试剂的作用，详细试剂一般也有不同的比例配方，在针对性上就有了更好的效果。PH7.67.47.27.0H2O1000WOO10001000NaCl8.58.58.58.5Na2HPO42.22.22.22.2NaH2PO40.10.20.30.4候IlWw蝮冲#M5mM500ml铁辄化钾的质肽为08231g电极扫描他用为-0208Vs亶铁气化”震冲溶波5mM500ml亚铁便化钾的质量为092OOg另：其它各种另PH值的0.2MPB(100ml)配方:PH0.2MNaH2PO4(ml)0.2MNa2HPO4(ml)5.89285.990106.087.712.36.185156.281.518.56.377.522.56.473.526.56.568.531.56.662.537.56.756.543.56.851496.945557.038627.133677.228727.323777.419ml81ml7.516847.613877.710.50.57.88.591.57.97938.05.394.7